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Extra resources for Advances In Biochemical Engineering Biotechnology. Thermal Biosensors Bioactivity Bioaffinity
1 High Resolution Thin-Film Thermistors . . . . . . . . 2 Miniaturized Enzyme Thermistors . . . . . . . . . . 3 Integrated Thermopiles . . . . . . . . . . . . . 62 Advances in Biochemical Engineering / Biotechnology, Vol. 64 Managing Editor: Th. Scheper © Springer-Verlag Berlin Heidelberg 1999 36 F. Lammers · Th. 4 Bio-Thermochips . . . . . . . . . . . . . . . 5 Compact Multichannel Enzyme Thermistors .
These are still being supplied in small series by Thermometric Co, Järfalla, Sweden or ABT, Lund. Figure 1 schematically shows the ET setup. It consists of an external aluminium cylinder that is 38 F. Lammers · Th. Scheper Fig. 1. 01°C). An inner aluminium cylinder contains two fastenings for a measuring and reference thermistor. A box filled with polyurethane foam insulates the aluminum cylinders. Samples are injected via the FIA-principle and pumped to the ET. 8 mm inner diameter). The tubes are connected with gold capillaries (good heat exchange) with fixed thermistors (type: GB42JM65, 16 kW at 25 °C; Fenwal Electronics, Framingham, MA, USA) and interchangeable columns containing the immobilized enzyme.
Here, medium concentrations up to 10 g/l are used (Amici et al. 1967). The ET is set up for L-asparagine analysis by columns of immobilized aparagine (200 units). 5 and 100 mmol/l. 6. 05 M Tris/HCl as well. The latter might be interesting for samples with high magnesium content. In this case, potassium phosphate buffer is not suitable because magnesium phosphate causes problems in FIA-systems. Especially in mammalian cell cultivations, the aminoacid L-glutamine and glucose represent the most important energy sources.
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