By Afaf Kamal-Eldin, Jan Pokorny
Lipid oxidation, notwithstanding researched because the starting of the twentieth century, nonetheless supplies no entire and passable info at the composition of oxidized lipids. One vital issue contributing to those gaps in our wisdom approximately lipid oxidation pertains to the shortages in analytical method. Analytical equipment compatible for oxidized lipids have been usually reviewed within the final decade, yet generally from the element of selection of person oxidized lipid sessions, comparable to peroxides, aldehydes, polar lipids, or polymers. during this publication, they're handled from the viewpoint of sorts of analytical tools used, together with varied volumetric tools, UV-visible spectrometric equipment, excessive functionality size-exclusion chromatography, nuclear magnetic resonance spectroscopy, electron spin resonance spectroscopy, and differential scanning calorimetry. research of Lipid Oxidation is vital for additional advancements in analytical technique and hyphenated thoughts, with which extra realizing of the response kinetics, mechanism, and implications will occur.
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1B). Monostearin is used as the internal standard because MG are normally in negligible, not detectable amounts in fats and oils. The nonpolar fraction is eluted with 15 mL of hexane:diethyl ether 90:10. A second fraction containing polar compounds and the internal standard is eluted with 15 mL of diethyl ether. , TLC to determine the efficiency of the separation and HPSEC. Fractions of polar compounds are analyzed by HPSEC under the same conditions previously described. Precision, accuracy, and recovery data were determined.
Because the reagent is added in great excess, only one of the two amine groups reacts. The absorbance of the condensation product is measured at the maximum at 350 nm. Condensation products with 2-alkenals and 2,4alkadienals have higher absorbances because of longer-chain conjugated double bonds. The method was modified using a more suitable solvent mixture, one in which samples were more soluble (Pokorn´y and Janíc˘ek 1966). The absorbance is measured at 430 nm. Because of good repeatability and reproducibility, the benzidine method was tested by the Fat and Oil Commission of IUPAC, and accepted as a standard method (Paquot and Hautfenne 1985).
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Analysis of lipid oxidation by Afaf Kamal-Eldin, Jan Pokorny